Bull semen sex-sorting using innovative technology Project leader Ilga Šematoviča Team of project Olga Ponomarjova Duration 2022 - 2025 Research focus 1. Implementation of the "One Health" concept and species interaction studies in the context of climate change Source of funding National grant Project partners Latvia University of Life Sciences and TechnologiesRiga Technical UniversitySIA Griezes LejniekiKurzemes CMAS/Dimedium Latvija ASSigulda CMASEvija EihvaldeMāra Mangale Description of project The aim of research Bull sperm sex-sorting using innovative technology for targeted female or male offspring. The tasks of project: To investigate the relationship between the dielectric potential of sex-differentiated spermatozoa and the efficiency of sex-sorting (at different frequencies, in different environments in microflow under computer vision control)To test the sex-sorted sperm within the project in vivo for fertilisation and sex-sorting accuracy by performing artificial insemination of cowsTo test the sex-sorted sperm within the project for in vitro fertilisation of oocytes by fertilising oocytes for embryo cultivation in the laboratoryThe work will be based on dielectrophoresis, but as a control, antibody detection with a flow cytometer will be used to verify the quality of sex-sorting, as well as the results in vivo and in vitro Results In progress
