Characterization and dynamics of seed-associated microbiome in weed soil seed-bank Project leader Jevgenija Ņečajeva Team of project Viktorija Zagorska Maksims Fiļipovičs Inta Jakobija Duration 2021 - 2023 Research focus 2. Preservation of biological diversity in agro- and forest biocenoses, ecosystem protection and services Source of funding National research programs Project partners Latvian Biomedical Research and Study Centre (BMC)External expert from LU - Vizma NikolajevaExternal expert from LU -Māris SeņkovsExternal expert from LU -Anete Borodušķe Description of project The aim of research Characterization and dynamics of seed-associated microbiome in weed soil seed-bankThe tasks of project: To determine the persistence of seeds in soil and to characterize seed properties, soil conditions and microbiological factors that influence the rate of seed decay a seed burial experiment will be established in two different locationsTo collect seeds in three years in different sites, to bury and exhume at intervals to test seed viability and germinationTo identify taxonomic profiles of bacterial and fungal species associated with the buried seeds using Next Generation Sequencing technologyTo test the ability of the seeds with different characteristics to withstand seed pathogens in laboratory conditions Results Epiphytic and Endophytic fungi colonizing seeds of two Poaceae weed species and Fusarium spp. seed degradation potential in vitro / Jevgenija Ņečajeva, Anete Borodušķe, Vizma Nikolajeva, Māris Seņkovs, Ineta Kalniņa, Ance Roga, Edmunds Skinderskis, Dāvids Fridmanis // Microorganisms. Vol. 11(1) (2023), article number 184. https://doi.org/10.3390/microorganisms11010184Characterisation and dynamics of the fungal communities of Avena fatua and Echinochloa crus-galli seeds in soil seed bank / Jevgenija Ņečajeva, Anete Borodušķe, Vizma Nikolajeva, Māris Seņkovs, Maksims Fiļipovičs, Inta Jakobija, Ineta Kalniņa, Ance Roga, Edmunds Skinderskis, Dita Gudrā, Dāvids Fridmanis // Weed Research. Nr. 64(4) (2024), p.310-320. https://doi.org/10.1111/wre.12651